The purpose of this study was to investigate the gender-related differencesin mathematics teaching anxiety. In March 2008, a Likert-type questionnaire wasadministered to 368 pre-service American mathematics teachers, residents of thestate of North Dakota (USA). In the sample 58.3% of the pre-service elementarymathematics teachers were females and 41.7 % were males. Likewise, 54.4% of thepre-service high school mathematics teachers were females and 45.6 % of themwere males. An independent samples t-test with alpha level of .05 was used in theanalysis of data. The results revealed no statistically significance in the genderrelateddifference in mathematics teaching anxiety between male and female preservicemathematics teachers. There was also no gender difference in teachinganxiety between males and females in the groups of middles schools and highschool teachers.